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. 2013 Dec 13;8(12):e83318. doi: 10.1371/journal.pone.0083318

Figure 8. PTEN expression in U87MG cells affects the PI3K class I/AKT/mTOR signaling pathway.

Figure 8

U87MG cells expressing WT-PTEN, C124S-PTEN or G129E-PTEN and mock-treated were incubated in high (H) and low (L) proteolysis media for 2 h. Total lysates were analyzed by Western blot using phospho-specific antibodies that recognize AKT-Thr308 (P-AKT-T) and AKT-Ser473 (P-AKT-S), p70S6K and 4-EBP1 and their respective pan-antibodies. The bands corresponding to the phosphorylated proteins, obtained from three different experiments, were densitometred and normalized to the corresponding total protein in the same sample. Results are shown on the histograms below the representative Western blots. Data are presented as percentage of the values in mock-treated (control) cells incubated in low proteolysis medium (L) and are means ± SD of three different experiments. Stars indicate statistically significant differences from the respective control values under high and low proteolysis conditions at *p<0.05 and ***p<0.005.