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. 2013 Dec 13;8(12):e83294. doi: 10.1371/journal.pone.0083294

Figure 2. Overexpression of miR-126 inhibited the EndMT process of EPCs induced by TGFβ1.

Figure 2

(A) The mRNA expression of progenitor cell markers (CD31, CD34, CD133, and KDR), (B) endothelial cell markers (VEGF, Flt-1, eNOS, and iNOS), and (C) mesenchymal cell markers (α-SMA, sm22-α, and myocardin) was determined by using qRT-PCR. (D) Protein expression of α-SMA was detected by immunofluorescence staining. EPCs induced by TGFβ1 were transfected with a miR-126-expressing lentiviral vector, an empty vector (lentivector), or neither, and EPCs without any treatment were used as the normal control. EPCs infected with an empty vector were used as the Lenti-miR control. Data shown are representative of 3 independent experiments. **, P < 0.01 compared with the Lenti-miR control.