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. 2013 Dec 13;8(12):e84306. doi: 10.1371/journal.pone.0084306

Figure 1. ATPase Assay of breast cell membranes treated with cardiac glycosides; (A) ouabain, (B) digitoxin, and (C) bufalin.

Figure 1

The Na,K-ATPase activity in total membranes isolated from non-tumorous 184D, 184A, and MCF10A (10A) cells, as well as cancerous MDA-MB-231 (MDA), MCF7, and MCF10CA1 (CA1) cells is inhibited in a concentration dependent manner for all cells types. Na,K-ATPase activity (µmol Pi liberated per mg protein per hour) was determined and displayed as the percentage of activity relative to the untreated sample for each cell type. There was no statistically significant difference in activity between normal and tumor cells treated with similar concentrations of ouabain, digitoxin, or bufalin (p>0.05).