Figure 2. Binding activity of Wisteria lectins to immobilized multivalent oligosaccharides and glycoproteins by glycan array analysis.

(A) Structures of polyacrylamide-based oligosaccharides and glycoproteins used for glycan array analysis. All oligosaccharides and glycoproteins used in this experiment (98 in total) are shown in Figure S1. Symbols corresponding to each monosaccharide are shown in the panel. Thin and thick bars represent alpha- and beta-linkages, respectively. Glycosidic linkage positions are shown by the numbers on the lower side of the panel. (B) Binding of each Wisteria lectin measured by an evanescent field-activated fluorescence scanner is shown. Oligosaccharides and glycoproteins are classified based on terminal sugar residues and indicated at the top of the upper panel. The data shown are the mean ± SD from three independent spots.