Figure 6. Direct administration of EETs and sEHI into the brain delay onset of PTZ induced seizures.

Bar graph of percent change in onset of clonic (black bars) and tonic (gray bars) seizures following intracerebroventricular administration of EpFAs, sEHI and diazepam (n = 6–14mice/group). Methyl ester EpFAs, sEHI and diazepam were dissolved in PEG400 and 1 µL was given by i.c.v. route to lightly anesthesized mice in the amounts indicated on x-axis. Thirty minutes after treatments PTZ assay was performed. Among the treatment groups, only diazepam was effective in delaying the onset of clonic seizures (Kruskal-Wallis One Way ANOVA on Ranks followed by Dunn's multiple comparison, *p≤0.05). Diazepam, EET-methyl esters and high dose of sEHI and were effective in delaying the onset of tonic seizures (Kruskal-Wallis One Way ANOVA on Ranks followed by Dunn's multiple comparison, **p≤0.05).