Table 2. The effects of allopregnenolone was significantly enhanced by sEHI and partially antagonized by finasteride (One Way ANOVA followed by Student Newman Keuls post hoc analysis TUPS+Allo vs TUPS, *p = 0.016, TUPS+Allo vs Allo, **p = 0.007, TUPS vs TUPS+FIN, #p = 0.02).
compound (mg/kg)+pentylenetetrazole | mean time to clonic seizure in seconds (SEM) | mean time to tonic seizure in seconds (SEM)§ | protected from tonic/total |
Vehicle (PEG400) | 257 (31) | 425 (45) | 0/12 |
TUPS (1) | 516 (102) | 809 (95)* | 8/18 |
Finasteride (10) | 204 (38) | 619 (80) | 0/8 |
TUPS+Finasteride (1+10) | 185 (19)# | 630 (66) | 3/8 |
Allopregnenolone (1) | 161 (24) | 587 (145)** | 0/4 |
Allopregnenolone (3) | 397 (16) | 850 (40) | 1/4 |
TUPS+Allopregnenolone (1+1) | 537 (113) | 1663 (478) | 3/4 |
Finasteride was administered 2 h, TUPS was administered 1 h, allopregnenolone, 10 min prior to PTZ.
§Observation period was extended to 45 min to increase the dynamic range of the assay.