Table 1. Bisphosphonate soaking solutions for long term study.

Silk-HA films were soaked in solutions which contained 82 to 800 µg clodronate per film or 7 to 136 µg alendronate per film. Following soaking, the amounts of bisphosphonates remaining in the soaking solution were measured and the amount bound per film was calculated.

		Osteoclasts		Osteoblasts		Co-cultures		Average
		Low	High	Low	High	Low	High
Clodronate	µg/film soaking solution	400	800	82	410	246	615
	Percentage bound to film	16%	15%	22%	15%	21%	18%	18%
	µg/film bound	64	119	18	63	52	113
Alendronate	µg/film soaking solution	68	136	7	14	37	75
	Percentage bound to film	26%	26%	34%	27%	32%	25%	29%
	µg/film bound	18	36	2.4	3.8	12	19