Annexin V and propidium staining in LLC-SOD1 and LLC-PK1 cells with or without MitoTEMPO following 2h-1h ATP-DR. A: Annexin V and propidium staining in LLC-PK1 (top panel) and LLC-SOD1 (lower panel). B: Quantitation of Annexin V fluorescence. C: The number of propidium iodide positive cells per field. SOD1 and MitoTEMPO significantly reduced Annexin V green fluorescence and the percentage of propidium positive cells. N=5–12; *, P < 0.05, LLC-DR vs. SOD-DR, SF, SOD-MitoTEMPO; ¶, P < 0.01, LLC-DR vs. SOD-MitoTEMPO; ‡, P < 0.001vs. SF. Scale bar = 60 µm.