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. 2013 Dec;14(12):1070–1083. doi: 10.1631/jzus.B1300094

Table 1.

Primers used for cloning, quantification, and subsellular localization of PpAPX

Gene Primer (5′–3′) Product size (bp)
Gene cloning
PpcAPX1 Forward: ACATGGGGCACTCACTCTCC 1 070
Reverse: GCTTTATTCAATCTACAAGG
PpcAPX2 Forward: ACATGGGGGTGCCACTCCATAG 1 070
Reverse: GAAGTGAAAATTACAGTAT
PpcAPX3 Forward: TTAGATATCCATTTTGATGTG 1 163
Reverse: ACCTCTGACATTACATTCGT
PppAPX Forward: AAAACCACATCTGTCCAGCT 1 155
Reverse: CATGTCACTATGTCCGTCGT
PpsAPX Forward: ATCTGTCCATTTTCCAAATC 1 577
Reverse: TTGATAGCAATGTAATTCTCAT
PptAPX Forward: GCCCCACCATTGGGGTGGAA 1 009
Reverse: GGAAATGCAACATTACAAGG

Quantification
PpcAPX1 Forward: GGAGAACCAAAAGGGATGATG 204
Reverse: GAATTGAAAATGACGGCTTTA
PpcAPX2 Forward: CGCGCCTTGCTTTTGTTT 192
Reverse: TAATACTGTAATTTTCACTTC
PpcAPX3 Forward: TCCAGTCTTTCGCCCTCT 132
Reverse: CTACAAATCACAATCCCTTA
PppAPX Forward: CGATACTGGCACAAGGAG 179
Reverse: ATTCGTAAGGGATCAAGG
PpsAPX Forward: CATGCTTACTTGTGCCATCTG 206
Reverse: AGAGGCTTATCTGGGCTTCC
PptAPX Forward: CTTTGGGCCAACAAATCAAT 201
Reverse: GACGCAGTTTTGAAGAGATGC

Subcellular localization
PpcAPX1 Forward: GGGGTACCACATGGGGCACTCACTCTCCATCGC 833
Reverse: CGGGATCCGGCCTCAGCAAACCCGAGCTCAGAG
PpsAPX Forward: GGGGTACCAGCCATCTGTCCATTTTCCA 1 233
Reverse: CGGGATCCCTCCTTGCCAGATGAGTATTTGGC