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. 2013 Oct 23;2(12):1300–1312. doi: 10.1242/bio.20136346

Fig. 2. UNC-40::GFP is localized at the sites of axon outgrowth.

Fig. 2.

Photomicrographs of the localization of UNC-40::GFP in the HSN neuron during the early stages of axon formation. UNC-40::GFP localization is associated with the sites of axon outgrowth. (A and B) An HSN neuron was imaged over a 1 h interval in the L2 stage. During this period a single broad leading edge forms ventrally. (A) Strong and uniform UNC-40::GFP fluorescence is observed on the left side (arrow) and at a leading ventral edge. (B) After 1 hour, strong and uniform UNC-40::GFP fluorescence is observed to the right (arrow), whereas the fluorescence on the left appears less uniform and intense. (C–E) During the L3 stage multiple neurites develop. Images show a progression of outgrowth. We note that the expression of UNC-40::GFP appears to exacerbate branching. See figure 1 in Adler et al. for a comparison to wild-type animals (Adler et al., 2006). Ventral is down and anterior is to the left. Scale bar: 5 µm.