Fig. 1. A comparison of TALEN scaffold structures.

(A) A schematic representation of TAL, ΔNΔC, ΔNΔC-ELD/KKR and ΔNΔC-ELD-S/KKR-S. (B) A comparison of TALEN scaffold amino acid sequences. The amino acid exchanges in the nuclease domain of ΔNΔC-ELD/KKR and ΔNΔC-ELD-S/KKR-S are underlined. (A,B) The shared amino acids in the N-terminal domain and C-terminal domain of TALEN are indicated with blue and yellow boxes, respectively. The nuclease domain of FokI is indicated with a red box. A purple triangle denotes the insertion-site of the DNA binding domain.