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. 2013 Nov 6;2(12):1364–1370. doi: 10.1242/bio.20136676

Fig. 2. The toxicity of TALEN mRNAs in X. tropicalis embryos.

Fig. 2.

(A) Morphologically normal embryos (Normal) with a loss of pigmentation in the retina after injection of ΔNΔC-ELD/KKR-Tyr I mRNAs. (B) A slightly deformed embryo (Slightly) that had not been injected with any mRNA. A small edema is indicated with a white arrowhead. (C) A severely deformed embryo (Severely) injected with ΔNΔC-ELD/KKR-Tyr I mRNAs. (D–I) Percentages of normal (blue), slightly deformed (yellow), severely deformed (red) and dead (black) embryos at NF-stage 35/36 (D,E,G,H) or NF-stage 24/25 (F,I). Embryos were injected with 400 pg (D,G), 80 pg (E,H) or 0 pg (control) of mRNAs encoding TAL, ΔNΔC, ΔNΔC-ELD/KKR or ΔNΔC-ELD-S/KKR-S TALEN for the Tyr I (D,E) or Tyr II (G,H) sites. (F,I) Embryos were injected with 400 pg of mRNA encoding TAL, ΔNΔC or ΔNΔC-ELD/KKR for the Tyr I left or right target site (F) and the Tyr II left or right target site (I). The number of embryos is indicated at the top of each column. The statistical significance compared to the control (a) or embryos injected with ΔNΔC-ELD/KKR mRNA (b) was assessed using a Steel-Dwass test. P<0.05. Scale bars: 1 mm.