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. 2013 Nov 28;2013:462152. doi: 10.1155/2013/462152

Figure 1.

Figure 1

Expression of Netrin-1 and the corresponding receptors in the mouse thymus and thymic cell types. (a) RT-PCR detection of Netrin-1 and Unc5b mRNA expression in the indicated cells. (b) This depicts a section of mouse thymus for the immunofluorescent staining of Keratin 8 (red) and Netrin-1 (green). The microscopic field shows that both molecules are largely colocalized in the thymus. Bars in the above and bottom panels are 100 μm and 25 μm, respectively. ((c) and (d)) RT-PCR detection of other receptors' mRNA expression in the indicated cells. Brain: positive control; TSC: thymic stromal cell; OP9: bone-marrow-derived stromal cell; MTEC: medullary thymic epithelial cell; DN: double negative thymocyte; DP: double positive thymocyte; CD4SP: CD4 single positive thymocyte; CD8SP: CD8 single positive thymocyte; H2O: negative control.