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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 May 2;33(7):10.1161/ATVBAHA.112.300922. doi: 10.1161/ATVBAHA.112.300922

Figure 4.

Figure 4

Small GTP-binding protein GDP dissociation stimulator (SmgGDS) small interfering RNA (siRNA) abolishes Rac1 degradation and anti-oxidant effects of atorvastatin in human umbilical venous endothelial cells (HUVECs). A, Western blot analysis of total cell lysate of HUVECs transfected with mock or SmgGDS siRNA for 72 hours. Atorvastatin was added during the last 24 hours (n=3). B, The effect of proteasome inhibitor (MG-132) on atorvastatin-induced Rac1 reduction in the nuclear fraction (n=3). C, Representative dichlorofluorescein staining of HUVECs after preincubation with atorvastatin (1 μmol/L) for 24 hours followed by 3 hours incubation with 1 μmol/L angiotensin II (AngII). D, Densitometric analysis (n=8). Results are expressed as mean±SEM. N.S. indicates not significant.