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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Circ Res. 2012 Dec 19;112(3):10.1161/CIRCRESAHA.112.273375. doi: 10.1161/CIRCRESAHA.112.273375

Fig 2. Cell dissociation impairs bioenergetics.

Fig 2

(A) 18FDG uptake and (B) ATP levels in 18–24 hr adherent and 1hr suspended CDCs following dissociation using trypsin(C) OCR and ECAR are significantly reduced in suspended CDCs (D) Re-plating after suspension for 1hr and 6hrs results in restoration of cellular metabolism, when assessed at 24hrs (n=6) (E) Oligomycin reduces OCR without affecting ATP levels in adherent CDCs (n=3) (F) Iodoacetate but not Oligomycin reduced ATP levels in adherent CDCs, indicating dependence on glycolysis for ATP generation (n=6)