Table 2.

ANS binding and lipid binding parameters of apoA-I variants.

	ANS	Parameters of binding of apoA-I variants to PC SUV at 25°C
	F.I.a	Kd	Bmax	ΔH	ΔGb	ΔSc
		µg/ml	amino acids/mol PC	kcal/mol	kcal/mol	cal/mol/K
Wild-type	1.0	2.6 ± 1.3	0.42 ± 0.05	−92.6 ± 5.3d	−12.0 ± 0.4	−270
Δ191–220	0.67	59 ± 23	0.24 ± 0.05	−60.4 ± 2.4	−10.1 ± 0.3	−169
Δ223–243	0.57	> 100 d	N.D. e	−40.6 ± 1.9 d	−9.9 d	−104 d

^aF.I. ; Fluorescence intensity.

^bThe Gibbs free energy was calculated according to ΔG = −RT ln 55.5 (1/K_d).

^cThe entropy of binding was calculated from ΔG = ΔH − TΔS.

^dData are added from [21].

^eN.D. ; Not determined.