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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 13;51(2):10.1016/j.freeradbiomed.2011.04.011. doi: 10.1016/j.freeradbiomed.2011.04.011

Fig. 2.

Fig. 2

Plots of observed rate constants of various intermediates that formed upon mixing of met-Hb against increasing concentration of HOCl. The observed rates of Fe(III)-OCl complex formation was monitored by following the decrease in the absorbance at 405 nm (Panel A). The observed rate constant of the buildup of compound II was followed by monitoring the increase in absorbance at 420 nm (Panel B). The observed rate constant of heme destruction was monitored by following the decrease in absorbance at 405 and 420 nm (Panel C). The experiments were carried out as in Fig. 1. The rates plotted are the average of three or four separate experiments.