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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 13;51(2):10.1016/j.freeradbiomed.2011.04.011. doi: 10.1016/j.freeradbiomed.2011.04.011

Fig. 5.

Fig. 5

In-gel heme staining confirms that HOCl treatment causes heme destruction from hemoglobin. Hb (6.25 µM) was treated with 160 fold of HOCl for 10 minutes at room temperature and in-gel heme staining was performed by o-dianisidine-H2O2 method, as detailed in Materials and Methods section (Panel A). Non-reducing SDS PAGE analysis of the same samples as in stained with coomassie blue for protein (Panel B). The data are representative of three independent experiments.