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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 13;51(2):10.1016/j.freeradbiomed.2011.04.011. doi: 10.1016/j.freeradbiomed.2011.04.011

Fig. 7.

Fig. 7

Release of free iron from hemoglobin following treatment with HOCl. 6.25 µM of Hb was treated with different molar ratios of HOCl (0 to 320 fold) for 10 minutes at room temperature. Free iron was assayed colorimetrically using ferrozine (for details see Materials and Methods section). The above result is an average of three independent experiments and the error bars represents the standard error of measurement.