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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 13;51(2):10.1016/j.freeradbiomed.2011.04.011. doi: 10.1016/j.freeradbiomed.2011.04.011

Fig. 9.

Fig. 9

Treatment of human RBC with HOCl leads to the generation of fluorescent heme degradation products in the RBC cytosol. Isolated human RBC adjusted to Hb concentration of 12.5 µM was treated with a range of HOCl:Hb molar ratios (0 to 160 fold HOCl) and analyzed by HPLC, as detailed in the Materials and Methods section. The fluorescent detector was set at excitation 321 nm and emission 465 nm. The molar ratio of the HOCl:Hb used for treatment is mentioned in inset of each panel. The above set of chromatograms is a representative of three independent experiments.