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. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: Int J Biochem Cell Biol. 2013 Sep 23;45(12):10.1016/j.biocel.2013.09.007. doi: 10.1016/j.biocel.2013.09.007

The miR-15 family regulates the expression of Bcl-w in MSC. Panel A. TargetScan shows that 3’ UTR of Bcl-w contains conserved miR-15 family binding sites. Panel B. The expression of miR-195 in the cells of 293TN transfected with miR-195 and miR-NC. Panel B: Relative luciferase activity of Bcl-w in 293TN cells after transfection with miR-195 and miR-NC, respectively. Panels D and E: Bcl-w protein in MSC^GATA-4 and MSC^Null, as measured by semi-quantitative western blot in normal (D) and hypoxic (E) conditions for 72 hours. Panel F: Bcl-w protein expression in MSC^GATA-4 transfected with miR-195 or miR-NC.

The miR-15 family regulates the expression of Bcl-w in MSC. Panel A. TargetScan shows that 3’ UTR of Bcl-w contains conserved miR-15 family binding sites. Panel B. The expression of miR-195 in the cells of 293TN transfected with miR-195 and miR-NC. Panel B: Relative luciferase activity of Bcl-w in 293TN cells after transfection with miR-195 and miR-NC, respectively. Panels D and E: Bcl-w protein in MSC^GATA-4 and MSC^Null, as measured by semi-quantitative western blot in normal (D) and hypoxic (E) conditions for 72 hours. Panel F: Bcl-w protein expression in MSC^GATA-4 transfected with miR-195 or miR-NC.