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. 2013 Nov 23;4:25–32. doi: 10.1016/j.fob.2013.11.003

Fig. 5.

Fig. 5

Phenotypes associated with ectopic overexpression of castor bean LEC2 in Arabidopsis transgenic plants. (A) Series of 8-week-old transgenic 35S:RcLEC2 plants (T1 generation) and levels of RcLEC2 transgene compared with that of wild-type plants (WT). Levels of RcACT2 transcript were used to normalize loading. A bushy phenotype of 12-week-old T1 transgenic plant #2 is shown in the inset. (B) Abnormal seedling phenotype in T2 transgenic plants germinated and grown in solid MS medium. The broader-than-normal hypocotyls of 35S:RcLEC2 seedlings stained strongly with Sudan Red 7B, indicating the accumulation of higher levels of oil than are found in wild-type (WT) seedlings. Scale bar in (B) represents 1 mm. (C) Phenotypes of T2 transgenic plants compared with WT plant. (D) Phenotypes of T3 transgenic plants compared with WT plants.