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. 2013 Nov 23;4:25–32. doi: 10.1016/j.fob.2013.11.003

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. 7 — Representatives of 35S:RcLEC2 Arabidopsis transgenic plants and expression of seed master regulator TFs’ genes in rosette leaves. (A) Growth retardation phenotype in T4 generation of 35S:RcLEC2 Arabidopsis transgenic plants compared with wild-type plants on 3 weeks. (B) RT-PCR analyses from leaves of wild-type (WT) and transgenic 35S:RcLEC2 plants (T4 generation). Two different individual plants of WT and two different lines of 35S:RcLEC2 transgenic plants were used for RT-PCR. Expression of the RcLEC2 transgene does not strongly induce expression of Arabidopsis LEC2 (At1g28300) but does induce expression of Arabidopsis LEC1 (At1g21970), L1L (At5g47670), FUS3 (At3g26790), ABI3 (At3g24650), and WRI1 (At3g54320). Three biological replicates showed the same expression pattern. The Arabidopsis ACTIN2 gene, AtACT2, was used as a control. 1 kb M indicates 1-kb DNA size marker.