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. Author manuscript; available in PMC: 2013 Dec 16.
Published in final edited form as: Cardiovasc Res. 2012 Mar 8;94(3):408–417. doi: 10.1093/cvr/cvs117

Figure 4.

Figure 4

Cardiac energy metabolism of Opa1+/+ and Opa1+/− mice. (A) Oxygen consumption rate of skinned ventricular fibers in the absence (Vo) or presence of 2mM ADP (Vmax) with 10mM glutamate and 4mM malate (complex I, CI), 15mM succinate (both CI and complex II CI+II), and after complex I inhibition with 2mM amytal (CII). (B) Acceptor control ratio. (C) Apparent Km of oxygen consumption for ADP with (+Cr) or without (−Cr) 12 mM creatine. (D) Oxygen consumption rate with 2 mM ADP and 4 mM malate and 0.1mM octanoyl-carnitine. (E) mRNA levels of proteins involved in mitochondrial biogenesis, glucose transport, and lipid utilization. (F) Citrate synthase (CS), complex I and total creatine kinase (CK) activities. (G–H) Efficacy of CK and mitochondria (DANC) to support SERCA (G) or myosin ATPase (H) in permeabilized fibers. *p<0.05 versus OPA1+/+ ***p<0.001 versus OPA1+/+