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. Author manuscript; available in PMC: 2013 Dec 16.
Published in final edited form as: Science. 2010 Jul 2;329(5987):10.1126/science.1187945. doi: 10.1126/science.1187945

Figure 3. Inhibition of BER and the consequences for DNA demethylation in zygotes.

Figure 3

a,c Small molecule PARP inhibitor 3AB and, APE1 inhibitor (CRT0044876) impede progression of DNA demethylation as detected by 5mC staining. b,d. Quantification of 5mC staining. The values represent a ratio between 5mC signal from paternal pronuclei relative to the signal from maternal pronuclei. Statistical analysis was by Student’s t-test. e. Bisulphite analysis of Line1 repetitive elements. The values show the percentage of methylated CpGs. Each line represents a unique DNA clone. Filled and open circles represent methylated and unmethylated CpGs, respectively. Scale bar 10μM. ♀ – maternal pronucleus, ♂ – paternal pronucleus, pb – polar body.