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. Author manuscript; available in PMC: 2013 Dec 16.
Published in final edited form as: Nature. 2009 Oct 29;461(7268):10.1038/nature08534. doi: 10.1038/nature08534

Table 1.

Derivation of rESC lines from mouse postimplantation embryos. The embryos were isolated from E5.5 - E7.5 129/sv or ROSA129/Sv females mated with Oct4-ΔPE-GFP transgenic males. The epiblast part was dissected and digested into single cell suspension. cEpi colonies emerged after 4 - 7 days in culture, and rESC were 20 observed after 14 - 35 days.

Transgenic lines Stage of embryos No. of embryos No. of
cEpi lines		 No. of
rESC lines
Oct4-ΔPE-GFP E5.5 22 14/22 (64%) 6/22 (27%)
Oct4-ΔPE-GFP E6.5 17 10/17 (59%) 4/17 (24%)
Rosa-LacZ/Oct4-ΔPE-GFP E6.5 28 22/28 (79%) 10/28 (36%)
Oct4-ΔPE-GFP E7.5 23 7/23 (30%) 5/23 (22%)