Figure 3.

Pulmonary hypertension (PH) lung bronchus-associated lymphoid tissues (BALTs) are bound by T cells by peripheral node addressin (PNAd). BALTs in experimental PH bind dye-labeled immunomagnetic bead sorted spleen and thymic rat CD3⁺ T cells (B and C), but control lung sections (A) do not. The T-cell adhesion may be largely attributed to binding to PNAd in BALTs (white arrows) because preincubation of lung sections with PNAd antibody attenuated T-cell adhesion to the BALT but not the lung parenchyma (D, yellow arrows). Representative images from tissue-cell incubations taken from lung sections from n = 6 animals per group, with two to three sections per rat from three independent experiments. Bars = 20 μm. All binding experiments were performed in triplicate. A = airway; MCT = monocrotaline.