Figure 1.

Chimeric antigen receptor (CAR)-redirected activation of T cells and cytokine-induced killer (CIK) cells. T cells and CIK cells from the same healthy donor were engineered with a carcinoembryonic antigen (CEA)-specific CD28-ζ CAR and coincubated (2.5 × 10³ − 2 × 10⁴ cells/well) for 48 hours with CEA⁺ LS 174T and CEA⁻ Colo320 target cells (2 × 10⁴ cells/well), respectively. Cytolysis was determined by an XTT-based colorimetric assay and culture supernatants were analyzed for interferon- (IFN-γ) and interleukin-2 (IL-2) by enzyme-linked immunosorbent assay. Numbers represent mean values of triplicates ± SD. A representative assay is shown. Statistical analyses were performed using Student's t-test. *P < 0.05.