Figure 6.

Intravenous injection of AAV9 in adult SOD1^G37R mice targets astrocytes and motor neurons within the spinal cord. (a–h) Immunofluorescence analysis revealed neuronal as well as glial transduction in both (a–d) AAV9-CB-GFP– and (e-h) AAV9-SOD1-shRNA–treated mice. (i–p) Human SOD1 levels appeared reduced in (o) AAV9-SOD1-shRNA treated mice compared with (k) AAV9-GFP treated mice. Bar = 100 µm. ChAT, choline acetyltransferase; GFP, green fluorescent protein; GFAP, glial fibrillary acidic protein; SOD1, superoxide dismutase 1.