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Extracted ion chromatogram (EIC) of the signature peptide NLLGLIEAK (485.82+). (a): W0.1 = 3.6 sec (485.82+) from 300 attomole on-line digested ProGRP separated and detected in the novel nanoproteomic platform with PLOT peptide separation. (b): W0.1 = 30 sec (485.82+) from 2,500 attomoles ProGRP, peptides separated on a conventional microbore column according to Torsetnes et al.22.
