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. 2013 Dec 16;3:3512. doi: 10.1038/srep03512

Figure 4. Sod3 levels in Aβ flies and effects of Sod3 RNAi on Sod3 expression, locomotion and survival.

Figure 4

(a–c). Levels of Sod3 transcripts in head RNA from Aβ (elavGAL4 > UAS-Aβ42 × 2) and control (elavGAL4/+) female mated flies at day 20 measured by qRT-PCR and plotted relative to Act5C mRNA levels, in arbitrary units, represented relative to levels in control (set to 1). (a) Total Sod3 mRNA levels (primers against A, B, D, E common transcript). (b) Sod3-RD mRNA levels. (c) Sod3-RE mRNA levels. (d–f), Levels of Sod3 transcripts in head RNA from Control (elavGAL4/+), Aβarc (elavGAL4 > UAS-Aβ42arc), Control + Sod3 RNAi and Aβarc + Sod3 RNAi female mated flies at day 7 measured by qRT-PCR and plotted relative to Act5C mRNA levels, in arbitrary units, represented relative to mRNA levels in Control (set to 1). qRT-PCR analysis: n = 3 replicates, mean ± s.e.m. plotted. mRNA levels between groups compared using two-tailed Student's t-test (f-test for equal variance). (d) Total Sod3 mRNA levels. (e) Sod3-RD mRNA levels. (f) Sod3-RE mRNA levels. (g) Climbing performance of Control (elavGAL4/+), Aβarc (elavGAL4 > UAS-Aβ42arc), Control + Sod3 RNAi and Aβarc + Sod3 RNAi mated females at different time points at 24°C. n = 3 (3 replicates, 10 flies/replicate). Performance indices (see Methods) between Control and Control + Sod3 RNAi and Aβarc and Aβarc + Sod3 RNAi were compared at each time-point using two-tailed Student's t-test (f-test for equal variance). (h) Survival curves of Aβarc (n = 105, median = 31, P < 0.0001 vs. Control) and Aβarc + Sod3 RNAi (n = 97, median survival = 35, P < 0.0001 vs. Aβarc) mated females at 25°C. (i) Survival curves of Control (n = 93; median survival = 66) and Control + Sod3 RNAi (n = 102; m = 66, P = 0.5280 vs. Control) mated females at 25°C. Comparison of survival curves was carried out using the Log-rank test. P values: *, P < 0.05; **, P < 0.01; ***, P < 0.0001.