Fig. 2.

Purification of EK and stability of the enzyme. (A) BENAC and (B) IMAC of EK_C122S_His5 after in vitro folding and autoactivation. L, load; F, flow-through; W1 and W2, washes; E1–3, elutions; M, molecular mass marker. All SDS-PAGE samples were prepared and run under nonreducing conditions. (C) EK stability during storage for 32 days at room temperature as judged from its activity against Bz-PFR-pNA.