Fig. 3.

Enzymatic activity of refolded EK and specific cleavage of EK targets. (A) Specific activities of EK_C122S_His5 and porcine EK (GenScript, Piscataway, NJ). Each value represents the mean of three measurements. (B) KLK4 was incubated without EK (∅) or with the protease at molar ratios ranging from 1:250 to 1:4000. (C) pro-uPA was incubated with EK at a molar ratio of 1:500. All SDS-PAGE samples were prepared under nonreducing conditions. M, molecular mass marker.