Table 2.

Yields during purification of EK_C122S_His5. Step numbers refer to Fig. 1.

Step	Amounta	Yield (per L culture)a	Yield (%)b
1 Cell pellet wet weight	35 ± 4 g	4.9 ± 0.5 g	–
2 IBs (after third wash)	18 ± 2 g	2.6 ± 0.2 g	–
2 IBs (after dialysis against citrate buffer)	16 ± 1 g	2.2 ± 0.1 g	100
6 BENAC eluate	28 ± 8 mg	3.9 ± 1.1 mg	0.2
7 Final product	12 ± 3 mg	1.7 ± 0.4 mg	0.1

^aMean values and standard deviations from three folding experiments.

^bRelative to weight of solubilized IBs (after dialysis against citrate buffer).