Table 2. List of genes used in this study.
The zebra finch cDNA fragments are listed with their corresponding region of the gene (base pair), GenBank accession number or the clones used, the clone ID, database source, and polymerase for antisense and sense riboprobes.
| Gene | Molecular Function | GenBank ID | GenBank Accession Number of Clone ID |
Enrichment of Brain Regions |
Polymerase for Antisense/Sense Riboprobes |
|---|---|---|---|---|---|
| COUPTF2 | Chicken ovalbumin upstream promoter-TF2 |
AB542998.1 | AB542998 (1–545 bp) | Nidopallium | T7/SP6 |
| D1B | Dopamine receptor 1B |
AB372108.1 | AB372108 (1-625 bp) | Striatum | T7/SP6 |
| DLX1 | Distal-less homeobox 1 |
CK306520 | CUGI 32K10 | Subpallium | T3/T7 |
| DLX6 | Distal-less homeobox 6 |
EH120087 | SBTD 0063P0006H11 | Subpallium (Striatum; Pallidum; POA) |
T3/T7 |
| EMX2 | Empty spiracles homeobox 2 |
XM_002187442 |
HM633193 (170 bp) |
Pallial ventricular zone |
SP6/T7 |
| ER81 | Ets-related protein 81 | DV582566 | SBTD 0064P0002B03 | Arcopallium; Hippocampus |
T3/T7 |
| FOXP1 | Forkhead box P1 | NM_001076698 |
AY549152 (1544-1711 bp) |
Mesopallium; Striatum |
T7/SP6 |
| GRIN2D | NMDA receptor 2D | AB042759.1 | AB042759 (1-521 bp) | Subpallium (Striatum; Pallidum; POA); Midbrain; Brainstem |
SP6/T7 |
| LHX8 | LIM homeobox 8 | EH122589 | SBTD 0057P0002H01 | Subpallium (Striatum; Pallidum; POA) |
T3/T7 |
| LHX9 | LIM homeobox 9, zinc finger |
XM_002193946 | AB542999 (1-467 bp) | Arcopallium; Hippocampus |
SP6/T7 |
| NKX2.1 | NK2 homeobox 2.1 | XM_002199787 |
HM633194 (1-637 bp) |
Subpallium (Pallidum) |
T7/SP6 |
| OTX2 | Orthodenticle homeobox 2 |
DV583144 | SBTD 0064P0001D02 | Forebrain-midbr ain boundary |
T3/T7 |
| PAX6 | Paired box 6 | DV580106 | SBTD 0063P0027B10 | Pallial ventricular zone |
T3/T7 |
| PPAPDC1A | Phosphatidic acid phosphatase 2 domain containing 1A |
CK305549 | CUGI 27G14 | Hyerpallium; Nidopallium |
T3/T7 |
| ROR-ß | RAR-related orphan receptor beta |
XM_002190956 |
XM_002190956 (702-1102 bp) |
Pallial sensory neurons (L2, E, and B) |
SP6/T7 |
| SEMA6A | Semaphorin-6A | CK308635 | CUGI P45I15 | Hyperpallium; Nidopallium; Striatum |
T3/T7 |