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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Exp Eye Res. 2013 Sep 25;116:10.1016/j.exer.2013.09.007. doi: 10.1016/j.exer.2013.09.007

Fig. 1.

Fig. 1

Alpha A-crystallin was undetectable by western analysis in MO knockdown embryos through four days post fertilization (dpf). Separate groups of 1-4 cell stage embryos were either left uninjected (U), or injected with morpholinos against αA-crystallin (αA) or a control morpholino (C) that does not recognize any zebrafish mRNA. Protein from the heads of embryos at three, four and five dpf were probed with antibodies against αA- and βB1-crystallin and detected with a chemiluminescent secondary antibody.