Fig. 3.

Knockdown of αA-crystallin produced no noticeable histological effects on lens development. A) Embryos on days two, three and four post fertilization were fixed, cryosectioned and stained with hematoxylin and eosin. These sections indicate no alterations in lens or retinal anatomy. Images are representative of at least three embryos examined for each timepoint, with none showing irregularities in lens development. B) Knockdown of AQP0a, which was already known to produce lens cataract by 3 dpf, produced a range of phenotypes including normal lenses and those with indentation (arrows) and persistent fiber cell nuclei. Three of six examined embryos showed some irregularities in lens development.