αA crystallin knockdown does not induce cataract formation. Representative z-plane (A-C) and 3D projections (D-F) of reflectance confocal image series taken of embryos injected with buffer only (A,D), αA crystallin MOs (B,E) or AQP0a MO (Positive control) (C,F). Arrows indicate cataracts, seen only in AQP0a injected MOs. (G) Percentage of embryos with cataracts (only one eye imaged per embryo), n≥7 for each condition. Error bars indicate standard deviation; two-tailed t-test used to measure statistical significance.