Table 1.

Sequences of primers for gene mutation and amplification studies

Primer name	Primer sequence (5′->3′)	Hybridization temperature	PCR fragment size
(M) BRAF exon 11 F	TCCCTCTCAGGCATAAGGTAA	55 °C	313 bp
(M) BRAF exon 11 R	CGAACAGTGAATATTTCCTTTGAT	55 °C
(M) BRAF exon 15 F	TCATAATGCTTGCTCTGATAGGA	55 °C	224 bp
(M) BRAF exon 15 R	GGCCAAAAATTTAATCAGTGCA	55 °C
(M) NRAS exon 2 F	GAACCAAATGGAAGGTCACA	55 °C	301 bp
(M) NRAS exon 2 R	TGGGTAAAGATGATCCGACA	55 °C
(M) NRAS exon 3 F	GGTGAAACCTGTTTGTTGGA	55 °C	272 bp
(M) NRAS exon 3 R	AACCTAAAACCAACTCTTCCCA	55 °C
(M) KIT exon 11 F	GATCTATTTTTCCCTTTCTC	55 °C	174 bp
(M) KIT exon 11 R	AGCCCCTGTTTCATACTGAC	55 °C
(M) KIT exon 13 F	CATCAGTTTGCCAGTTGTGC	55 °C	295 bp
(M) KIT exon 13 R	AGCAAGAGAGAACAACAGTCTGG	55 °C
(M) KIT exon 17 F	TCATTCAAGGCGTACTTTTG	55 °C	350 bp
(M) KIT exon 17 R	TCGAAAGTTGAAACTAAAAATCC	55 °C
(M) KIT exon 18 F	CATTTCAGCAACAGCAGCAT	60 °C	287 bp
(M) KIT exon 18 R	CAAGGAAGCAGGACACCAAT	60 °C
(A) ACTIN F	CATGGTGCATCTCTGCCTTACA	60 °C	76 bp
(A) ACTIN R	ACAGCCTGGATAGCAACGTACA	60 °C
(A) GADPH F	GCCCCCGGTTTCTATAAATTG	60 °C	94 bp
(A) GADPH R	CTGGCGACGCAAAAGAAGAT	60 °C
(A) KIT F	TCCTCAAACAGGCATAGATTTCC	60 °C	69 bp
(A) KIT R	TGTGGATAGCATGCCTTGGA	60 °C
(A) MDM2 F	AGGACATCTTATGGCCTGCTTTAC	60 °C	63 bp
(A) MDM2 R	GGGCAGGGCTTATTCCTTTT	60 °C
(A) CDK4 F	ATTGCATCGTTCACCGAGATC	60 °C	65 bp
(A) CDK4 R	CTTGACTGTTCCACCACTTGTCA	60 °C
(A) CCDN1 F	CGTGGCCTCTAAGATGAAGGA	60 °C	62 bp
(A) CCDN1 R	CGGTGTAGATGCACAGCTTCTC	60 °C
(A) MITF F	TGTCACTGATCCACTCCTTTCCT	60 °C	59 bp
(A) MITF R	TCCGGCTGCTTGTTTTGG	60 °C

Abbreviations : F, forward; R, reverse; bp, base pair, M, primer used for mutation studies; A, primer used for amplification studies