Table 4.
KIT, CCND1, CDK4, MDM2 and MITF gene copy number assessment and Ckit and cyclin D1 protein expression
| Case | KIT gene copy number | cKit protein in situ | cKit protein invasive | CCND1 gene copy number | CCND1 Amplification (FISH) | Cyclin D1 protein in situ | Cyclin D1 protein invasive | CDK4 gene copy number | MDM2 gene copy number | MITF gene copy number |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 4 | 1+ | 5+ | 1 | − | − | 1+ | 3 | 3 | 3 |
| 2 | 3 | 5+ | 0 | 2 | ND | 1++ | 2++ | 3 | 2 | 4 |
| 3 | 2 | NE | 0 | 3 | ND | NE | 0 | 3 | 2 | 4 |
| 4 | 5 | NE | 1+ | 7 | + | NE | 8+++ | 4 | 5 | 4 |
| 5 | 3 | 5++ | 0 | 9 | ND | 3++ | 3+++ | 2 | 1 | 3 |
| 6 | 3 | 3++ | 0 | 3 | ND | 2++ | 5++ | 4 | 3 | 4 |
| 7 | 1 | NE | 0 | 5 | ND | NE | 0 | 3 | 2 | 3 |
| 8 | 1 | NE | 0 | 4 | ND | NE | 0 | 2 | 2 | 5 |
| 9 | 2 | 5+ | 0 | 13 | ND | 0 | 1+ | 8 | 4 | 8 |
| 10 | 8 | 5++ | 5++ | 1 | ND | 0 | 0 | 2 | 2 | 3 |
| 11 | 2 | 5++ | 5+ | 12 | + | 0 | 0 | 3 | 2 | 3 |
| 12 | ND | 1+++ | 1+++ | ND | ND | ND | ND | ND | ND | ND |
| 14 | 2 | 9+++ | 9+++ | 3 | − | 5+++ | 7+++ | 3 | 2 | 3 |
| 15 | 4 | 0 | 0 | 7 | + | − | 1+ | 4 | 4 | 4 |
| 16 | 2 | NE | 5+ | 2 | − | NE | 5+++ | 3 | 2 | 2 |
| 17 | 1 | 10+++ | 5++ | 2 | ND | 7+++ | 8+++ | 2 | 2 | 2 |
NOTE : For immunohistochemical analyzes, the proportion of positive cells is expressed as 1 for 10%, 2 for 20%, up to 10 for 100%, and the staining intensity as + for low, ++ for moderate and +++ for high.
Abbreviations : ND, not done; NE, non evaluable.