Skip to main content
. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 11;191(12):10.4049/jimmunol.1301169. doi: 10.4049/jimmunol.1301169

Fig 5. B cells in the IRF8−/−HEL-Tg mice are more resistant to antigen stimulation induced apoptosis than their counterparts in the IRF8+/+HEL-Tg mice.

Fig 5

Splenocytes were isolated from IRF8−/−HEL-Tg and IRF8+/+HEL-Tg mice through negative selection. The isolated cells were plated into 96-well plate in triplicate at 5x105 cells per well in presence of increasing concentrations of BAFF (ng/ml). After 72h, the percentages of live cells were analyzed via PI staining. P=0.45 (Linear regression analysis). B) The isolated splenocytes were plated into 96-well plate in triplicate in presence of increasing concentrations of HEL (ng/ml). After 48h, the percentages of live cells were analyzed by FACS. P= 0.02 (linear regression analysis). C) The isolated splenocytes were incubated with HEL (200 ng /ml) in presence of increasing concentrations of BAFF (ng/ml). Two days later, the percentages of live cells were analyzed by FACS. P=0.03(linear regression analysis).