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(A) Sfp-catalyzed modification of PCP, ACP or ybbR tag at a specific Ser residue by CoA-SR conjugates. (B) Using Sfp or related PPTases for direct site-specific protein immobilization via surface immobilized CoA as a substrate and a protein of interest containing a genetically encoded carrier protein or a ybbR tag. (C) Labeling cell surface proteins containing ACP, PCP or a ybbR tag using PPTase.
