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Schematic representation of site-specific labeling of proteins using transglutaminase (TGase) catalyzed ligation of cadaverine-functionalized probes (green star) to cell surface proteins recombinantly fused to a Q-tag (yellow rectangle). TGase site-specifically incorporates a probe into the protein by ligating the glutamine of the Q-tag to the amine probe and forms a new amide bond between the two while releasing ammonia into solution.
