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. 2011 Apr 2;3(2):141–170. doi: 10.3390/pharmaceutics3020141

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — IncuCyte™ kinetic imaging platform and applications. Kinetic monitoring and quantification of (A) cell confluence, (B) cell migration following wounding of a monolayer of HT1080 fibrosarcoma cells. Data represents wound width (μm) at sequential time points quantified using the IncuCyte migration application module. (C) Angiogenesis in a human primary co-culture model of endothelial (HUVEC) and fibroblast cells where HUVECs have been labeled with GFP. Data represents cells treated in co-culture with VEGFR2 inhibitor (0.1 μM) or vehicle control for 10 days. Tubule area quantified using the IncuCyte angiogenesis algorithm. (D) Caspase-mediated apoptosis in a human micovascular endothelial cell line (HMEC-1) treated with Staurosporine (0.3 μM) and cultured in the presence of NucView™ caspase substrate. Caspase positive cells quantified using the IncuCyte object counting algorithm. n = 3 (error bars are standard deviation).

Figure 1. — IncuCyte™ kinetic imaging platform and applications. Kinetic monitoring and quantification of (A) cell confluence, (B) cell migration following wounding of a monolayer of HT1080 fibrosarcoma cells. Data represents wound width (μm) at sequential time points quantified using the IncuCyte migration application module. (C) Angiogenesis in a human primary co-culture model of endothelial (HUVEC) and fibroblast cells where HUVECs have been labeled with GFP. Data represents cells treated in co-culture with VEGFR2 inhibitor (0.1 μM) or vehicle control for 10 days. Tubule area quantified using the IncuCyte angiogenesis algorithm. (D) Caspase-mediated apoptosis in a human micovascular endothelial cell line (HMEC-1) treated with Staurosporine (0.3 μM) and cultured in the presence of NucView™ caspase substrate. Caspase positive cells quantified using the IncuCyte object counting algorithm. n = 3 (error bars are standard deviation).