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. 2011 Apr 2;3(2):141–170. doi: 10.3390/pharmaceutics3020141

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 6. — Intravital in vivo imaging and Fluorescence recovery after photobleaching (FRAP) to monitor E-cadherin dynamics. (A) Example of an optical window chamber containing a tumor implanted on the back of a CD-1 nude mouse. Inset: confocal image of GFP-E-cadherin expressing tumor cells acquired using optical window chambers, a major blood vessel is outlined. Scale bar 50 μm. (B) A representative FRAP recovery curve after photobleaching of a region of GFP-E-cadherin at the plasma membrane in vivo.