Figure 7.

Intravital in vivo photoswitching to monitor tumor invasion. (A) Images from a time series acquired from a GFP expressing squamous cell carcinoma (SCC) xenograft expressing wild-type FAK (green: GFP, purple: confocal reflectance of extracellular tissue), scale bar 50 μm. Lower panel represents co-localized images from the time-series showing tracked single cell movement within tumors (green, 0 seconds; red, 100 seconds; blue, 300 seconds). White arrows indicate direction of cell movement. (B) Images represent Dendra2 labeled SCC FAK-wild-type expressing cells imaged using optical window chambers at 0, 6, and 24 hours post photo-switching (green, unswitched; red, switched). Scale bar 100 μm. Graph represents quantification of the total area covered by red fluorescence at sequential time-point following photo-switching.