Figure 2.

An overview of integrated strategies for small proteins prediction. It is challenge to differentiate meaningful gene-coding sORFs from inutile sORFs because the shorter the protein sequence, the greater the probability of error rate of detection. First we suggest splitting the annotation of SPs from other proteins. Second, it is better to combine both in silico algorithms and evidence-based analysis. Then merge the two parts of results and get two sets of SPs as follows. The strict validated SPs are those validated by both methods, while other validated SPs are those only validated by either in silico algorithms or evidence-based analysis.