Fig. 1.

Phosphorylation of Sec2p increases binding to the exocyst component Sec15p in vitro. (Left) GST-Sec2p was purified from bacteria (Sec2b) or from yeast (Sec2y). Sec2y was treated with the phosphatase CIP or was left untreated. His₆-Sec15p was purified from bacteria, eluted, and incubated with GST-Sec2p immobilized on glutathione beads. Bound Sec15p was detected with anti-Sec15p antibody, and GST-Sec2p was detected with anti-GST antibody. (Right) The percentage of bound Sec15p was calculated and is indicated. The mean and SD of three different experiments are shown. *P < 0.002; **P < 0.05, Student t test.