Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Phosphomimetic mutation of serines 186 and 188, but not 181, leads to increased binding of Sec15p and a corresponding decrease in binding of Ypt32p-GTP. (A–C) GST-Sec2p, His6-Sec15p, and His6-Ypt32p were purified from bacteria. Sec15p and Ypt32p were eluted. Ypt32p was preloaded with GTPγs and then was incubated with GST-Sec2p immobilized on glutathione beads. Bound proteins were detected with anti-Sec15p antibody or anti-Ypt32p antibody. GST-Sec2 was detected with anti-GST antibody.
