Fig. 1.

Orai1 expression in human skin and its role in keratinocytes differentiation. (A) immunohistochemical studies for the expression of Orai1, STIM1, IVL, KRT10, and FLG in human skin sections. Top Left shows the respective control for our stainings using secondary antibody only. Insets (Right) show enlarged images of the boxed regions. Different layers (Inset, Top Left) in epidermis are: (i) stratum basale; (ii) stratum spinosum; (iii) stratum granulosum; (iv) stratum lucidum; (v) stratum corneum. (Scale bars: 50 µm.) (B) Real-time quantitative PCR from three independent experiments showing the expression levels of Orai1, STIM1, and FLG in hPK kept in 0.07 mM Ca²⁺ (UhPK), or hPK cells treated with 1.7 mM Ca²⁺ during 1, 3, or 6 d (DhPK). Data were normalized compared with HPRT gene expression. Data represented are mean ± SEM; *P < 0.05; **P < 0.01, n = 3. (C) The effects of Orai1 knockdown on the expression of FLG, IVL, KRT1, and TGM1 transcripts induced by calcium switch (1.7 mM Ca²⁺, 24 h). Data were normalized compared with HPRT gene expression. Data represented are mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001; n = 3. (D) The expression of IVL and FLG in hPK kept in 0.07 mM Ca²⁺ induced by Orai1 knockdown for 24 h. Data were normalized compared with HPRT gene expression. Data represented are mean ± SEM; **P < 0.01; n = 3.